Tóm tắt

There is a renewed interest in the application of chitosan-based drug delivery systems over the last few years. Inrnthis study, the ionic gelation method was used to prepare chitosan-engaged tripolyphosphate ions, as the cross-linking molecule, (Chit-TPP) and concurrent loading of the biomolecules of the ethanolic extract of fennel,rnFoeniculum vulgare, seed (FEC@NBC). The samples were characterized by SEM, DLS, TGA, FTIR, XRD, GC–MS,rnand zeta potential, and their effects on the related hormonal and biochemical factors of the rats with polycysticrnovarian syndrome (PCOS) were assessed. The estradiol valerate-induced PCOS in female rats was confirmed byrnvaginal smear test and subsequent histological screening. The PCOS-induced rats were treated by fennel seedrnextract (FSX), Chit-TPP, and FEC@NBC. The process of treatment was monitored by measuring the serum levelsrnof testosterone, luteinizing hormone, follicle-stimulating hormone, insulin, glucose, high-density lipoproteinrncholesterol, total cholesterol, and total triglyceride after 16 days of treatment and compared with healthy controlrnand untreated PCOS-control groups. The FEC@NBC administration contributed to the remarkable hormonal,rnglucose, and lipid profile regulation in the rats with PCOS. The significance of FEC@NBC performance in dealingrnwith PCOS complications compared to that of the only extract could be resulted from the effective targetedrndelivery and stability of phytomolecules when encapsulated in Chit-TPP