Tóm tắt

Traditional Chinese medicine therapy, which can serve as adjuvant therapy for cancer treatment, has no obviousrnside effects on the human body. Geniposide (GEN), one of the main iridoid glycosides in gardenia fruit, has beenrnwidely reported to have anti-cancer effects. In this study, we aimed to inspect whether GEN could inhibitrnproliferation and promote the apoptosis of human breast cancer cells (MCF-7). In order to better predict thernefficacy of GEN, we have prepared the Cs/Gel composite scaffolds by 3D printing technology to mimic the MCF-7rncell growth microenvironment. The prepared Cs/Gel scaffold has good mechanical properties and biocompat-ibility, which can provide a more accurate platform for drug screening. The semi-inhibitory concentration (IC50)rnevaluated by CCK-8 assay was 16.06 mg/mL (24 h), 14.85 mg/mL (48 h), and 13.14 mg/mL (72 h). Afterrnexposed to GEN for 48 h, the cancer cell survival rate reduced from 69.15 ± 2.86% (13 mg/mL) torn20.97 ± 3.24% (16 mg/mL). Although the inhibitory effect was weaker in the 3D culture system, it alsornmanaged to inhibit cell proliferation and induce cell apoptosis. Besides, Live/Dead staining, Hematoxylin-Eosinrn(H&E) staining and SEM evaluation were also conducted to estimate the anti-cancer effect of GEN in 2D and 3Drncultures. The results indicate that GEN has an anti-cancer effect based on a time- and dose-dependent manner.