Tóm tắt

Colorimetric biosensors can be used to detect a particular analyte through color changes easily by nakedrneyes or simple portable optical detectors for quantitative measurement. Thus, it is highly attractive forrnpoint-of-care detections of harmful viruses to prevent potential pandemic outbreak, as antiviral medica-tion must be administered in a timely fashion. This review paper summaries existing and emerging tech-niques that can be employed to detect viruses through colorimetric assay design with detailed discussionrnof their sensing principles, performances as well as pros and cons, with an aim to provide guideline on thernselection of suitable colorimetric biosensors for detecting different species of viruses. Among the colori-metric methods for virus detections, loop-mediated isothermal amplification (LAMP) method is morernfavourable for its faster detection, high efficiency, cheaper cost, and more reliable with high reproduciblernassay results. Nanoparticle-based colorimetric biosensors, on the other hand, are most suitable to be fab-ricated into lateral flow or lab-on-a-chip devices, and can be coupled with LAMP or portable PCR systemsrnfor highly sensitive on-site detection of viruses, which is very critical for early diagnosis of virus infec-tions and to prevent outbreak in a swift and controlled manner