Tóm tắt

Biodegradable and injectable hydrogels derived from natural polysaccharides have attracted extensive attentionrnin biomedical applications due to their minimal invasiveness and ability to accommodate the irregular woundrnsurfaces. In this work, we report the development of an in-situ-injectable, self-healing, antibacterial, hemostatic,rnand biocompatible hydrogel derived from the hybrid of N,O-carboxymethyl chitosan (N,O-CMC) and oxidizedrnchondroitin sulfate (OCS), which did not require any chemical crosslinking. The N,O-CMC/OCS hydrogel couldrnbe readily produced under physiological conditions by varying the N,O-CMC-to-OCS ratio, relying on the Schiffrnbase reaction between the–NH–functional groups of N,O-CMC and the -CHO functional groups of OCS. Thernresults showed that the N,O-CMC2/OCS1 hydrogel had relatively long gelation time (133 s) and stable perfor-mances. The viability of NIH/3T3 cells and endothelial cells cultured with the N,O-CMC2/OCS1 hydrogel extractrnwas roughly 85%, which demonstrated its low cell toxicity. Besides, the N,O-CMC2/OCS1 hydrogel revealedrnexcellent antibacterial properties due to the inherent antibacterial ability of N,O-CMC. Importantly, the hydrogelrntightly adhered to the biological tissue and demonstrated excellent in vivo hemostatic performance. Our workrndescribing an injectable, self-healing, antibacterial, and hemostatic hydrogel derived from polysaccharides willrnlikely hold good potential in serving as an enabling wound dressing material.