Tóm tắt

Over the years, several in-vitro biosensing platforms have been developed for enhancing the maturation of the rncultured cells. However, most of the proposed platforms met with limited success due to its inability for live-cell rnimaging, complicated fabrication, and not being advantageous from an economic perspective due to a higher rnprice. To overcome the drawbacks of the current state-of-the-art, herein, we developed a next-generation stage- rntop incubator (STI) incorporated with nano grooves patterned PDMS diaphragm (NGPPD). The proposed device rnconsists of a miniatured STI, the NGPPD functional well plates, and a mechanical stimulator. A thin layer of gold rn(Au) is deposited on the NGPPD to enhanced myogenic differentiation, cell maturation, and cell-cell interactions. rnThe nano grooves are integrated on the PDMS surface to align the cardiomyocytes in the grooved direction rnduring the culture period. The cardiomyocytes cultivated on the Au-deposited NGPPD are stimulated topo-graphically and mechanically during the cultivation period. The enhanced cardiomyocytes maturation cultured rnon the Au-deposited NGPPD is experimentally demonstrated using immunofluorescence staining and PCR ana-lysis.