Tóm tắt

Objectives:To investigate the pulpal repair potential of an experimental zirconium-oxide containing tricalcium-silicate cement, referred to as‘TCS 50’.rnMaterials and methods:The effect of TCS 50 on viability, proliferation, migration, and odontoblastic differ-entiation of human dental pulp cells (HDPCs) was assessed using XTT assay,in-vitrowound healing assay and RT-PCR, respectively. Additionally, the pulp-capping potential was evaluated using a vital human tooth model.rnStatistical analysis was performed using non-parametric Kruskal-Wallis test and post-hoc test (Mann-WhitneyUrntest). The tests were performed at a significance level ofα= 0.05.rnResults:The effect of TCS 50 towards HDPCs was dose dependent. Undiluted TCS 50 extract showed no im-mediate adverse impact on cell viability (p > .05); however, it significantly inhibited proliferation and mi-gration of HDPCs (p < .05). A 25% diluted TCS 50 extract showed no significant effect on cell viability,rnproliferation or migration (p > .05), and it significantly enhanced odontoblastic differentiation of HDPCsrn(p< .05). In pulps capped with TCS 50 for both 2 and 4 weeks, H&E staining revealed a normal morphology ofrnpulp tissue; mineralized foci with cellular components entrapped in the matrix were formed underneath thernexposure site. Collagen I expression was weak within the matrix of mineralized foci, while the expression ofrnnestin was positive for entrapped cellular components within the mineralized foci, indicating that the formedrnmineralized foci corresponded to an initial form of reparative dentin formation.rnConclusion:TCS 50 is capable of generating an early pulp-healing reaction and therefore could serve as a pro-mising pulp-capping agent