Tóm tắt

The present study selected the predominant multi antibiotic-resistant diabetic foot ulcer (DFU) derived bacterialrnisolates such asPseudomonas aeruginosa(PA),Escherichia coli(EC),Staphylococcus aureus(SA) andBacillus subtilisrn(BS) and evaluated their response against the well-characterized Aerva lanata(AL) reduced multiple phyto-chemicals fabricated silver nanoparticles (AL-AgNPs). The overnight culture of DFU isolates was processed andrnsubjected to various studies such as antimicrobial activity, growth kinetics, biofilm disruption, reactive oxygenrnspecies (ROS), membrane leakage, membrane permeability, and damage and genotoxicity. The molecularrndocking of AL phytochemicals was also performed with bacterial enzyme DNA gyrase. Interestingly, AL-AgNPsrnwere produced the remarkable antibacterial effect against the resistant DFU isolates, which was closely similar tornthe effect of AL-AgNPs observed against the reference strains. The minimum inhibitory concentration (MIC) andrnminimum bactericidal concentration (MBC) of AL-AgNPs against the DFU isolates were found to be 5–15μg/mLrnand 10–20μg/mL, respectively. The AL-AgNPs were depicted a concentration-dependent growth inhibition ofrnDFU bacterial isolates. The MIC and MBC of AL-AgNPs were effectively destroyed the preformed biofilms of DFUrnisolates. Furthermore, the MBC of AL-AgNPs was displayed the increased intracellular ROS accumulation,rnmembrane leakage, permeability and damage, and genotoxicity in the DFU isolates. Additionally, thein silicornstudy revealed that the AL phytochemicals werefitted over the binding pocket of the DNA gyrase B subunit. Thernobserved results were confirmed that the negative impacts of the AL-AgNPs at the level of the membrane andrnintracellular components of DFU isolates.