Tóm tắt

Bone injuries and fractures generally take a long period to heal itself. To address this problem, bone tissue rnengineering (BTE) has gained significant research impetus. Among the several techniques used for scaffold rnfabrication, electrospinning ought to be the most promising technique for the development of the nanostructured rnscaffolds. The present study was carried out to fabricate an electrospun nanocomposite scaffold for BTE by using rngelatin, polycaprolactone (PCL), and nanohydroxyapatite (nHAp). To prepare Gelatin-PCL-nHAp nanocomposite rnscaffold: Gelatin-PCL blend was electrospun and then treated with nHAp (1 wt%) for different time periods. The rnfabricated nanocomposite scaffold was analysed by field emission scanning electron microscopy (FESEM) to rndetermine the fiber diameter and evaluate the fiber morphology. The Gelatin-PCL-nHAp nanocomposite scaffold- rn20 min exhibited the average fiber diameter of 615 ± 269 nm and average pore size 4.7 ± 1.04 μm, and also rnrevealed the presence of nHAp particles over the Gelatin-PCL scaffold surface. Further, X-ray diffraction (XRD), rnFourier Transform Infrared (FTIR) spectroscopy and thermogravimetric (TG) analysis also indicated the de-position of nHAp over the Gelatin-PCL scaffold surface. MTT assay and DNA quantification showed good via-bility and significant proliferation of human osteoblasts on Gelatin-PCL-nHAp nanocomposite scaffold. rnMoreover, cell-scaffold constructs illustrated efficient cellular attachment and adequately spread cells, and it also rndepicts characteristic polygonal morphology of osteoblasts over the Gelatin-PCL-nHAp nanocomposite scaffold. rnThus, the results of in-vitro analysis of electrospun nanocomposite scaffold suggest that the Gelatin-PCL-nHAp rnscaffold can be a potential candidate for BTE applications.