Tóm tắt

Recent advances in regenerative medicine have given hope in overcoming and rehabilitating complex medical rnconditions. In this regard, the biopolymer poly-ε-caprolactone (PCL) may be a promising candidate for tissue rnregeneration, despite lacking the essential bioactivity. The present study used PCL nanofibers (NFs) scaffold rndecorated with the extracellular matrix proteins fibronectin and laminin combined for neuronal regeneration. rnThe potential for the dual proteins to support neuronal cells and promote axonal growth was investigated. Two rnNFs scaffolds were produced with PLC concentrations of 12% or 15%. Under scanning electron microscopy, both rnscaffolds evidenced uniform diameter distribution in the range of 358 nm and 887 nm, respectively, with > 80% rnporosity. The Brunauer–Emmett–Teller (BET) test confirmed that the fabricated NFs mats had a high surface rnarea, especially for the 12% NFs with 652 mrn2rn/g compared to 254 mrn2rn/g for the 15% NFs. The proteins of interest rnwere successfully conjugated to the 12% PCL scaffold through chemical carbodiimide reaction as confirmed by rnFourier-transform infrared spectroscopy. The addition of fibronectin and laminin together was shown to be the rnmost favorable for cellular attachment and elongation of neuroblastoma SH-SY5Y cells compared to other for-mulations. Light microscopy revealed longer neurite outgrowth, higher cellular projected area, and lower shape rnindex for the cells cultured on the combined proteins conjugated fibers, indicating enhanced cellular spread on rnthe scaffold. This preliminary study suggests that PCL nanoscaffolding conjugated with matrix proteins can rnsupport neuronal cell viability and neurite growth.