Tóm tắt

To protect unwanted tissue adhesions occurring after surgeries, we aimed to fabricate an anti-adhesivefilm usingrncartilage acellular matrix (CAM) with anti-vascular inhibition activity. Additionally, to fabricate anti-adhesivernfilms with tunable swelling, mechanical, and biodegradation properties, a biodegradable polyester (PEP) withN-hydroxysuccinimide (NHS) in the chain end position was synthesized as a cross-linker. CAM/PEP (CP)films werernprepared with various CAM: PEP ratios in the wide size with repeatable reproducibility, and then, cross-linkedrnCP (Cx–CP) were obtained by the interpenetrating cross-linking reaction between the amine group on CAM andrnthe NHS group on PEP cross-linkers under thermal treatment. The biodegradation, wettability, swelling, andrnmechanical properties of the prepared anti-adhesive Cx–CPfilms were controlled by varying the CAM:PEP ratio.rnThe degradation half-life, contact angle, elastic moduli and toughness of Cx–CPfilms increased according to thernincreasing PEP content. Additionally, Cx–CPfilms significantly inhibits the attachment and proliferation ofrnHUVECs. Cx–CPfilm prepared by varying the CAM:PEP ratio can be tailored to meet individual requirements forrnin vivo injured tissues. In animal experiments, anti-adhesive Cx–CPfilms implanted between the peritoneal wallrnand the cecum significantly suppressed tissue adhesion between them. Additionally, good adhesion effect ob-served at anti-adhesivefilm maintained for proper time period at injured tissues. Taken together, in this work,rnwe successfully achieved strategy for the development of anti-adhesive barrier with tunable swelling, me-chanical, and biodegradation properties.